Hello BowForMe,
In your opening post, you said:
ESSP Secure Data creates an encryption 'keystore' file called 'premiumkey.dat' in C:\Users\USERNAME\AppData\Local\DESlock+\
If you delete this directory and the files within, then the encryption key is no longer available and your encrypted USB cannot be decrypted or accessed. Re-installing ESSP and enabling Secure Data will generate a new keystore file, which will NOT work for anything encrypted with a different key.
If we're lucky, and you still have the directories and files you deleted in the Recycle Bin, then you should be able to restore them and access your USB stick! If not, perhaps you have a Windows backup that you can restore where the DESlock+ directory and files are intact and can be restored to regain access to your USB.
Failing this, the data on the USB is inaccessible and you will need to format the device for future use.
Kind regards
Jay Pritchard
Encryption Technical Support Engineer III / Team Lead